Se diseñaron los biorreactores, utilizando principios de similitud; para la producción del alcohol etílico, se diseñó un biorreactor del tipo Lecho Empacado . Inmovilización de levaduras en residuos lignocelulósicos para la producción de etanol en biorreactor de lecho empacado. En las fermentaciones realizadas en los biorreactores de lecho empacado con el biocatalizador (soporte + levaduras), se logró obtener un aumento en la.

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Immobilization matrices Used as immobilization matrices were lignocellulosic materials, sugar cane bagasse, corn leaves, corn cobs and wood shavings, obtained as agro-industrial waste.

judith galvan bautista

The concentration of alcohol was determined by HPLC. This study is focused on the development of an immobilization process of yeast cells in waste lignocellulosic materials and their evaluation in the ethanol production by using packed bed bioreactors.

The fermentation time of 12 h.

In order to confirm immobilization and determine the differences between the carriers, we obtained images by scanning electronic microscopy SEM. Ethanol production began in the early hours of fermentation, but only provided an empadado accumulation by 6 hours after the process started.

Two different types of bioreactors were designed, using principles of similarity.

In the first column the carriers studied are listed corn leaves, wood shavings, corn cob and cane bagasse. The baseline immobilization was performed by following the yeast immobilization protocol. The adsorption in these carriers can occur by different mechanisms, such as mechanical adsorption and retention in the pores of the carriers, adherence to the areas for different types of bonds and immobilization due to hydrophobic or biochemical interactions between carriers and cells.

The absorbance reading was done with a spectrophotometer Spectronic Genesis 2, modelserial no. In this work, we evaluated the continuous production of ethanol in packed-bed reactors with empacadl cells immobilized on wood shaving, cane bagasse, corn leave, and corn cob lignocellulosic waste.


Applied and environmental microbiology, 68 7pp. The experimental design analysis using Design Expert software allowed us to establish that there is no significant statistical difference between treatments.

The parameters obtained in the batch process are shown in Table lexho. For the continuous process, we used a time of residence of 3 h and a flow of 1.

Bioresources Technology, 98 4. Effect on cell Surface Properties and Immobilization.

Most of these mechanisms are spontaneously generated during contact between cells and carrier, so adsorption is one of llecho simplest mechanisms to perform, both in a laboratory and industrial level. Subsequently, the bioreactor was filled with fresh culture medium and allowed to operate with recirculation for 12 hours [19].

Revista Facultad de Ingeniería

The detection by UV absorbance was set to nm. Figures 4 and 5 show the results obtained for the continuous fermentation carried out with sucrose. We used a column Supelco gel CH with the following conditions: Se evaluaron cuatro diferentes residuos de materiales lignocelulosicos: One of the more relevant biofuels for the substitution of fossil fuels is ethanol [1], which is mainly produced by alcoholic fermentation in batch processes.

We can see that the amount of immobilized cells varies depending on the treatment and the material. If that doesn’t help, please let us know. The established time of residence t was 3 h, and the reactor was operated this way for 6 times of residence 18 h. The results obtained have allowed us to establish the potential this lignocellulosic waste has as carriers for cell immobilization for the continuous production of ethanol, and the possibility of using glucose syrup as an alternative substrate.

Study of the effect of size and flow rate for each material To determine the effect on immobilization of flow rate and the carrier size, we performed a factorial experimental design with 2 factors and 2 levels, and a central point with three replicates.


We kindly thank the Biotechnology and Biotransformation Groups of the Universidad de Antioquia for their support.

Copy of biorreactor de lecho empacado by judith galvan bautista on Prezi

Asamblea Reactor Colocar los componentes del reactor de acuerdo con el esquema de la Figura 1. In additional experiments, we were able to establish that the carriers absorb some of the sugars in the culture medium, decreasing the amount of sugars available to be processed into alcohol, and llecho decreasing the performance of the fermentation.

Immobilization yeasts cells, bioethanol, biofuels, lignocellulosic carriers, continuous fermentation. Based on these results we chose the biomass quantification protocol modified dry weight treatmentthat uses NaOH 0.

To determine the size range of the carriers, we considered the bioreactor used and mass transfer criteria for packed bed columns. These results indicate that, despite of the total absence of sugars at the end of fermentation, not all of them were converted to ethanol. A similar value, Before starting the continuous process, we carried out the stages of cell immobilization and batch fermentation previously described.

Based on these results, the best choice of flow rate and size lfcho cell immobilization can be established considering the best operating conditions of the reactor, because the values of the studied ranges for flow rate and size have no effect on the amount of immobilized biomass in the carriers. Differences on the surface of the structures of the four materials can be found, these differences can lead to changes in yeast cell immobilization. The maximum theoretical yield of sucrose to ethanol is 0.